Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.
Identifieur interne : 000E33 ( Main/Exploration ); précédent : 000E32; suivant : 000E34Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.
Auteurs : A. Koch [Allemagne] ; C T Weigel ; G. SchulzSource :
- Gene [ 0378-1119 ] ; 1993.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Agaricales (enzymologie), Agaricales (génétique), Bactériophage T7 (génétique), Cellulase (génétique), Clonage moléculaire (MeSH), Deuteromycota (enzymologie), Deuteromycota (génétique), Données de séquences moléculaires (MeSH), Escherichia coli (génétique), Gènes fongiques (MeSH), Penicillium (enzymologie), Penicillium (génétique), Saccharomyces cerevisiae (enzymologie), Saccharomyces cerevisiae (génétique), Similitude de séquences d'acides aminés (MeSH), Similitude de séquences d'acides nucléiques (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Trichoderma (enzymologie), Trichoderma (génétique).
- MESH :
- enzymologie : Agaricales, Deuteromycota, Penicillium, Saccharomyces cerevisiae, Trichoderma.
- génétique : ADN fongique, Agaricales, Bactériophage T7, Cellulase, Deuteromycota, Escherichia coli, Penicillium, Saccharomyces cerevisiae, Trichoderma.
- Clonage moléculaire, Données de séquences moléculaires, Gènes fongiques, Similitude de séquences d'acides aminés, Similitude de séquences d'acides nucléiques, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Agaricales (enzymology), Agaricales (genetics), Amino Acid Sequence (MeSH), Bacteriophage T7 (genetics), Base Sequence (MeSH), Cellulase (genetics), Cloning, Molecular (MeSH), DNA, Fungal (genetics), Escherichia coli (genetics), Genes, Fungal (MeSH), Mitosporic Fungi (enzymology), Mitosporic Fungi (genetics), Molecular Sequence Data (MeSH), Penicillium (enzymology), Penicillium (genetics), Saccharomyces cerevisiae (enzymology), Saccharomyces cerevisiae (genetics), Sequence Homology, Amino Acid (MeSH), Sequence Homology, Nucleic Acid (MeSH), Trichoderma (enzymology), Trichoderma (genetics).
- MESH :
- chemical , genetics : Cellulase, DNA, Fungal.
- enzymology : Agaricales, Mitosporic Fungi, Penicillium, Saccharomyces cerevisiae, Trichoderma.
- genetics : Agaricales, Bacteriophage T7, Escherichia coli, Mitosporic Fungi, Penicillium, Saccharomyces cerevisiae, Trichoderma.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genes, Fungal, Molecular Sequence Data, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid.
Abstract
From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Trichoderma reesei cellulase-encoding gene probe (egl1). Both cDNAs have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. In the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase I of T. reesei and 70% homology with cellobiohydrolase I of T. reesei, Phanerochaete chrysosporium, and Humicola grisea. Expression of the 1.9-kb cDNA in the Escherichia coli T7 system led to the detection of a 57-kDa protein, in agreement with the theoretical value. Fusion to the promoter of the yeast phosphoglycerokinase-encoding gene led to efficient expression and partial secretion of the cDNA-encoded cellulase with cellobiohydrolase I activity in Saccharomyces cerevisiae.
DOI: 10.1016/0378-1119(93)90761-q
PubMed: 8440481
Affiliations:
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Schulz</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8440481</idno><idno type="pmid">8440481</idno><idno type="doi">10.1016/0378-1119(93)90761-q</idno><idno type="wicri:Area/Main/Corpus">000E23</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E23</idno><idno type="wicri:Area/Main/Curation">000E23</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E23</idno><idno type="wicri:Area/Main/Exploration">000E23</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.</title><author><name sortKey="Koch, A" sort="Koch, A" uniqKey="Koch A" first="A" last="Koch">A. Koch</name><affiliation wicri:level="1"><nlm:affiliation>Institut für Mikrobiologie, Humboldt-Universität zu Berlin, Kleinmachnow, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut für Mikrobiologie, Humboldt-Universität zu Berlin, Kleinmachnow</wicri:regionArea><wicri:noRegion>Kleinmachnow</wicri:noRegion><wicri:noRegion>Kleinmachnow</wicri:noRegion><wicri:noRegion>Kleinmachnow</wicri:noRegion></affiliation></author><author><name sortKey="Weigel, C T" sort="Weigel, C T" uniqKey="Weigel C" first="C T" last="Weigel">C T Weigel</name></author><author><name sortKey="Schulz, G" sort="Schulz, G" uniqKey="Schulz G" first="G" last="Schulz">G. Schulz</name></author></analytic><series><title level="j">Gene</title><idno type="ISSN">0378-1119</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (enzymology)</term><term>Agaricales (genetics)</term><term>Amino Acid Sequence (MeSH)</term><term>Bacteriophage T7 (genetics)</term><term>Base Sequence (MeSH)</term><term>Cellulase (genetics)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Fungal (genetics)</term><term>Escherichia coli (genetics)</term><term>Genes, Fungal (MeSH)</term><term>Mitosporic Fungi (enzymology)</term><term>Mitosporic Fungi (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Penicillium (enzymology)</term><term>Penicillium (genetics)</term><term>Saccharomyces cerevisiae (enzymology)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Sequence Homology, Nucleic Acid (MeSH)</term><term>Trichoderma (enzymology)</term><term>Trichoderma (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>Agaricales (enzymologie)</term><term>Agaricales (génétique)</term><term>Bactériophage T7 (génétique)</term><term>Cellulase (génétique)</term><term>Clonage moléculaire (MeSH)</term><term>Deuteromycota (enzymologie)</term><term>Deuteromycota (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Escherichia coli (génétique)</term><term>Gènes fongiques (MeSH)</term><term>Penicillium (enzymologie)</term><term>Penicillium (génétique)</term><term>Saccharomyces cerevisiae (enzymologie)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Similitude de séquences d'acides nucléiques (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Trichoderma (enzymologie)</term><term>Trichoderma (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cellulase</term><term>DNA, Fungal</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Agaricales</term><term>Deuteromycota</term><term>Penicillium</term><term>Saccharomyces cerevisiae</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Agaricales</term><term>Mitosporic Fungi</term><term>Penicillium</term><term>Saccharomyces cerevisiae</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agaricales</term><term>Bacteriophage T7</term><term>Escherichia coli</term><term>Mitosporic Fungi</term><term>Penicillium</term><term>Saccharomyces cerevisiae</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Agaricales</term><term>Bactériophage T7</term><term>Cellulase</term><term>Deuteromycota</term><term>Escherichia coli</term><term>Penicillium</term><term>Saccharomyces cerevisiae</term><term>Trichoderma</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Genes, Fungal</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Gènes fongiques</term><term>Similitude de séquences d'acides aminés</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Trichoderma reesei cellulase-encoding gene probe (egl1). Both cDNAs have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. In the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase I of T. reesei and 70% homology with cellobiohydrolase I of T. reesei, Phanerochaete chrysosporium, and Humicola grisea. Expression of the 1.9-kb cDNA in the Escherichia coli T7 system led to the detection of a 57-kDa protein, in agreement with the theoretical value. Fusion to the promoter of the yeast phosphoglycerokinase-encoding gene led to efficient expression and partial secretion of the cDNA-encoded cellulase with cellobiohydrolase I activity in Saccharomyces cerevisiae.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8440481</PMID><DateCompleted><Year>1993</Year><Month>03</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN><JournalIssue CitedMedium="Print"><Volume>124</Volume><Issue>1</Issue><PubDate><Year>1993</Year><Month>Feb</Month><Day>14</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.</ArticleTitle><Pagination><MedlinePgn>57-65</MedlinePgn></Pagination><Abstract><AbstractText>From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Trichoderma reesei cellulase-encoding gene probe (egl1). Both cDNAs have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. In the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase I of T. reesei and 70% homology with cellobiohydrolase I of T. reesei, Phanerochaete chrysosporium, and Humicola grisea. Expression of the 1.9-kb cDNA in the Escherichia coli T7 system led to the detection of a 57-kDa protein, in agreement with the theoretical value. Fusion to the promoter of the yeast phosphoglycerokinase-encoding gene led to efficient expression and partial secretion of the cDNA-encoded cellulase with cellobiohydrolase I activity in Saccharomyces cerevisiae.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Koch</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut für Mikrobiologie, Humboldt-Universität zu Berlin, Kleinmachnow, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Weigel</LastName><ForeName>C T</ForeName><Initials>CT</Initials></Author><Author ValidYN="Y"><LastName>Schulz</LastName><ForeName>G</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017123" MajorTopicYN="N">Bacteriophage T7</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005800" 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Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014242" MajorTopicYN="N">Trichoderma</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>2</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>2</Month><Day>14</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>2</Month><Day>14</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8440481</ArticleId><ArticleId IdType="pii">0378-1119(93)90761-Q</ArticleId><ArticleId IdType="doi">10.1016/0378-1119(93)90761-q</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Schulz, G" sort="Schulz, G" uniqKey="Schulz G" first="G" last="Schulz">G. Schulz</name><name sortKey="Weigel, C T" sort="Weigel, C T" uniqKey="Weigel C" first="C T" last="Weigel">C T Weigel</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Koch, A" sort="Koch, A" uniqKey="Koch A" first="A" last="Koch">A. Koch</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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